Fig. 5

Appb regulates blastoderm cell adhesiveness. Embryos of wild-type or appbmutant background were injected with red (R) or green (G) fluorescent dextran at the one-cell stage, dissociated and mixed 1:1 at sphere stage and co-cultured in fibronectin coated plates (A,B). Cluster formation started around 6 hours post dissociation (hpd) (C,D) and was allowed to proceed for 16 hpd before image analysis (E,F). Scale bar = 200 μm. Frequency distributions of scattering ‘S’ (G) and dipole moment ‘P’ (H) of red and green cells in clusters of wt/wt (black line) and wt/appb mut (grey line). Dashed lines mark interval for scattering. *p < 0.05.