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Fig. 4
Proliferation of EVL in wild-type and appb mutants at 4 hpf. Representative confocal images of the EVL in wild-type (A) and appb mutant (B) embryos immunostained for the mitotic marker phospho-Histone H3 (pHH3; green) and actin binding phalloidin (red) at 4 hpf. All nuclei were labeled with DAPI (blue). Number of pHH3-positive EVL nuclei shown in percentage of all EVL cells (C). Student’s t-test was performed on wild-type (n = 8) and appbmutant (n = 8). Scale bar, 100 μm.
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