ZFIN Image: Paatero et al., 2018, Fig. 2

Image

Figure Caption

Fig. 2 Active VE-cad and F-actin behavior of junction-based lamellipodia. a, b Still images from a movie (Supplementary Movie 2) of a VE-cad-Venus expressing embryo Tg(BAC(cdh5:cdh5-ts)), showing the DLAV at 30 hpf in inversed contrast. b A magnification of the inset in a. Arrows point to JBL. c, d Still images from a movie (Supplementary Movie 3) of a EGFP-UCHD expressing embryo (Tg(fli:Gal4ffubs3, UAS:EGFP-UCHDubs18)) showing the DLAV at 30 hpf in inversed contrast. d A magnification of the inset in c. Arrows point to JBL. e Scatter plot of quantitation of the duration of the JBL with the VE-cad-Venus transgene (n = 48 in 6 embryos) and EGFP-UCHD movies (n = 74 in 6 embryos), red line represents the median. f Quantitation of JBL angle in the DLAV in respect to the blood vessel axis (0°) using the EGFP-UCHD transgene (n = 103 from 6 embryos) or Cdh5-Venus transgene (n = 41 from 5 embryos). Scale bars 5 µm

Figure Data

Expression / Labeling details

Acknowledgments

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