Fig. 4

Fig. 4

a Representative stereo micrographs of macrophages in the whole tail region and quantification of the number of macrophages in this region. 3 dpf dram1^{∆19n/∆19n} and dram1^+/+/ mpeg1:mCherryF larvae were obtained from incrossed dram1^+/∆19n animals and the number of macrophages for each larva were counted before determining the genotype. Genotyping was performed by PCR and Sanger sequencing (≥28 larvae/group). Data are accumulated from two independent experiments and represented by scatter and boxplots as detailed in the “Methods” section. ns non-significant, *p < 0.05,**p < 0.01,***p < 0.001. b Representative stereo images of the whole tail of 3 dpf dram1^{∆19n/∆19n} and dram1^+/+ larvae following an immunohistochemical peroxidase activity detection protocol. The number of neutrophils in this region was quantified per individual larva (≥18 larvae/group). Data are accumulated from two independent experiments and represented by scatter and boxplots as detailed in the “Methods” section. ns non-significant, *p < 0.05,**p < 0.01,***p < 0.001. c Representative confocal micrographs of macrophages located in the blood circulation over the yolk sac of infected dram1^{∆19n/∆19n} and dram1^+/+ embryos in mpeg1:mCherryF background at 1 h post-infection (hpi). Scale bars, 10 μm. Quantification of phagocytosis of Mm by macrophages at 1 hpi. dram1^{∆19n/∆19n} and dram1^+/+ embryos in mpeg1:mCherryF background were infected with Mm at 30 hpf and fixed at 1 hpi. The percentage of macrophages having phagocytosed Mm clusters was determined per individual larva (≥16 larvae/ group). Results are accumulated from two independent experiments and represented by scatter and boxplots as detailed in the “Methods” section. ns non-significant, *p < 0.05,**p < 0.01,***p < 0.001.