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Figure 5

Timp4 and both Mmp11 paralogues are present in the MTJ at 28 hpf. High-resolution confocal micrographs of MTJs in the trunk skeletal musculature dorsal to the yolk extension of 28 hpf embryos stained with antibodies against (A) Mmp11a, (B) Mmp11b, (C) Timp4, and (D) Laminin (green) as well as anti-α-actinin (red) reveal that both Mmp11 paralogues are detectable in MTJs at this stage, and that they accumulate in the periphery of the MTJ (arrowheads), whereas Timp4 accumulates in the core of the MTJ. Scale bars are 10 µm. Anterior to the left in all images.

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Acknowledgments
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