Frequency of spontaneous contractions and Ca2+ transients in the trunk of zebrafish embryos (24–28 hpf). (A) Representative experiments showing contractions (measured by transmission light, upper panels) and Ca2+ transients (measured by bioluminescence, lower panels) in the same embryo expressing GA (left) or mitoGA (right). (B) Comparison between the average frequency of contractions observed by transmitted light (imaging at 4.2 Hz) and Ca2+ transients (imaging at 4.7 Hz) in the cytoplasm (GA, left) and mitochondria (mitoGA, right). A two-tailed paired t-test was used. Shown as mean ± SEM (n = 6 embryos).
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