Figure 5

Figure 5

Effect of inhibiting mitochondrial Ca²⁺ uptake on the frequency of contractions and Ca²⁺ transients in 24–28 hpf embryos. Effect of the uncoupler FCCP (1 µM) and the MCU inhibitor DS16570511 (100 µM) on the frequency of skeletal muscle contractions in the trunk in non-injected embryos (A), and on cytosolic (B), and mitochondrial (C) Ca²⁺ transients. In (B) and (C) the frequency and amplitude of Ca²⁺ transients are shown. A two-tailed paired t-test was used. Shown as mean ± SEM (n = 5 to 13 embryos). The graphs on the right are representative experiments. Statistical significance was considered for * p < 0.05, *** p < 0.001, **** p < 0.0001.