ZFIN Image: Djannatian et al., 2019, Fig. 4

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Figure Caption

Fig. 4

Loss of adhesive molecules results in myelin overgrowth in zebrafish. a Myelin sheaths in 10/11 dpf cntn1b^−/−mag^−/− fish show fluorescence intensity steps absent in wild-type fish. Fluorescence intensities along example sheaths. ‘a’ and ‘b’ represent mean fluorescence intensity levels. b Fluorescence intensity ratios (calculated ‘a’/’b’ according to panel ( a) in 11 representative cntn1b^−/−mag^−/− sheaths (n = 4) show two fold increase in fluorescence along the sheath in bright areas as compared to the neighboring regions. Median with interquartile range. c Length of sheaths with fluorescence intensity steps (n = 53 sheaths from 3 fish) vs. evenly labeled sheaths (n = 100 sheaths from 3 fish) in 10 dpf cntn1b^−/−mag^−/− fish (two-tailed Mann Whitney test, ***p < 0.0001, median with interquartile range). Sheaths were quantified from a more dorsal region compared to Fig. 2. d Expression of mbp:mCherry-CAAX in 11 dpf caspr^−/−mag^−/− fish reveals double myelin sheaths (asterisk) and loss of nodes of Ranvier (arrowhead). Fluorescence intensity plot along the upper myelinated axon (green = EGFP-CAAX, magenta = mCherry-CAAX). Dotted lines represent corresponding positions in image and plot. Images ( a, d) are maximum intensity projections of Tg(mbp:EGFP-CAAX) zebrafish dorsal spinal cord. Scale bars, 4 μm ( a), 10 μm ( d). Source data are provided as a Source Data file

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.