ZFIN Image: Cox et al., 2019, Fig. S6

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Figure Caption

Fig. S6

Crispr/Cas9 targeting of adam19b in Tg(3.2:egfp:hsp70:nsfB-mcherry.crest) larvae expressing mCherry in motor neurons and egfp in epibranchial sensory ganglia. Panels (A–C)represent control fish: (A) shows motor neurons and axons (mV, mVII and mX; red channel:hsp70:nsfB-mcherry.crest), (B) shows epibranchial sensory ganglia (green channel:3.2:egfp; sVII, sIX and sX are the projections of the facial (gVII), glossopharyngeal (gIX) and vagus (gX) ganglia, respectively) and (C) shows a composite of both images. Panels (D–F) represent Crispr/Cas9 targeting of adam19b. Panel (D) shows unaffected mV, mVII and mX motor neurons (red channel:hsp70:nsfB-mcherry.crest), (E) shows misrouted epibranchial ganglia projections (dotted white arrow; green channel:3.2:egfp) and (F) shows a composite of both images. All images are oriented anterior to the left and dorsal at top. Scale bars = 100 μm.

Acknowledgments

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