ZFIN Image: Kim et al., 2019, Fig. 5

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Fig. 5

Expression of genes involved in inflammation and apoptosis induced by Sirt1-knockout induced inflammation.

All images were obtained from 12-month-old zebrafish. (A) Immunohistochemical staining for myeloperoxidase (MPO). (B) ISH for NF-kB and (C) TNF-α expression. (D) TUNEL assay with 18-month old zebrafish organ sections. Wildtype organ section with few apoptotic cells and Sirt1^-/- organ section with numerous apoptotic cells, as per the TUNEL assay. (E) Survival curves of wildtype and Sirt1^-/- mutant zebrafish. Kaplan–Meier analysis showed poor survival of Sirt1^-/- compared to the wildtype. (n = 100 zebrafish in each group). Survival curves lasted for 540 days (observation started when zebrafish were 3 months old.) and were statistically compared using the Log-Rank Test. (F) Schematic diagram of the major findings of this study. Sirt1^-/—induced chronic inflammation led to the activation of oxidative stress and apoptosis. The continuous repetition of these events reduced the lifespan of zebrafish.

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Acknowledgments

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