Fig. 6
Fig. 6
Injury-induced expression is associated with regeneration. a Whole-mount in situ analysis of gene expression after fin amputation. Arrows point to the injury-induced expression at the amputation sites. TAB-5 wild-type embryos at 3 dpf were used for fin amputation, and the amputated embryos were fixed at 15 h post amputation for whole-mount in situ analysis. TAB-5 wild-type embryos at 4 dpf without amputation were used as the control. b Quantitative PCR analysis of gene expression during maternal stages (1-cell, 16-cell), maternal–zygotic transition (512-cell), and later stages (dome, 1 dpf, and 5 dpf). Beta-actin was used as internal reference. The sequences for the primers used for the probe synthesis and qPCR are listed in Suppl. Table 3