ZFIN Image: Shim et al., 2019, Fig. S2

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Figure Caption

Fig. S2

In vivo validation of candidate coronary artery disease (CAD) genes.

(a) The sequence of translation-blocking MOs targeting candidate CAD genes used for this study. (b) Tg(flk1:EGFP) zebrafish embryos were injected with morpholinos (MOs) for candidate CAD genes and compared with control MO-injected embryos (morphants). The majority of morphants, except for apod morphants, exhibit gross morphological abnormalities, including a small brain, heart edema, and curved tail, at 3 days post-fertilization (scale bar = 500 μm). (c) Diagrams show the representative heart defects, such as no asymmetry (midline) and reversed asymmetry between ventricle and atrium, at 3 days post-fertilization.

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Phenotype details

Acknowledgments

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