ZFIN Image: Nichols et al., 2019, Fig. 6

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Fig. 6

Invadopodia are cell autonomously required for spinal entry. a Confocal z-projection images taken from a 24-h time-lapse starting at 48 h post fertilization (hpf) of a Tg(sox10:lifeact-GFP); Tg(sox10:PA-Rac1-mcherry) zebrafish with both photoactivated and non-photoactivated Rac1. Orange box denotes dorsal root entry zone (DREZ). White arrow denotes growth cone. b Quantification of the number of PA-Rac1⁺ neurons that enter the spinal cord. Photoconverted neurons fail to enter the spinal cord. SEM is shown, n = 10 non-photoactivated dorsal root ganglia (DRG), n = 9 photoactivated DRG. c Quantification of the number of PA-Rac1⁺ neurons that form actin-invasive structures. Photoactivated neurons fail to form actin-invasive components. SEM is shown, n = 10 DRG. d Intensity profiles of growth cone Lifeact-GFP in neurons with photoactivated (n = 9) and non-photoactivated (n = 10) Rac-1. eDistance traveled by neurons with photoactivated (n = 9) and non-photoactivated (n = 10) Rac-1. f Confocal of two growth cones of a Tg(sox10:lifeact-GFP) animals stained for Cortactin showing co-localization of Cortactin with Lifeact-GFP foci at the DREZ. g Confocal images of a Tg(ngn1:gfp) animals stained for pSrc showing localization of pSrc in pioneer growth cones. Scale bars denote 10 µm in (a) and 5 µm in (f, g). Insets in (f, g) are single-plane confocal images

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