ZFIN Image: Sun et al., 2018, Fig. 1

Image

Figure Caption

Fig. 1

Maternal ybx1 mutant embryos exhibit severe morphological defects. (A) Generation of the ybx1 mutant allele using CRISPR/Cas9. Top: the gRNA target site (red arrow) within the first exon (E1). Grey boxes, white boxes and connecting lines represent the open reading frame, untranslated regions and introns, respectively. Middle: sequences of ybx1 WT and ybx1^tsu3d5i alleles near the gRNA target site (underlined) showing the deleted 3 bp (boxed) and the 5-bp insertion (red). Bottom: domains of Ybx1 WT protein and predicted mutant protein. APD, alanine/proline-rich domain; CSD, cold shock domain; CTD, C-terminal domain. (B,C) Loss of ybx1 mRNA in Mybx1 embryos revealed by qRT-PCR (B) and WISH (C). (D) Bright-field images showing the embryonic malformation of Mybx1 mutants in contrast to time-matched WT embryos. (E) Bright-field and fluorescent images of Mybx1 embryos expressing GFP-Ybx1. Mybx1; Tg(ef1α:GFP-ybx1) embryos were obtained by crossing Zybx1; Tg(ef1α:GFP-ybx1) females to WT males. Scale bars: 200 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development