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Fig. S7
ES supplementation rescues pigmentation of Ctr1 knockout zebrafish while equivalent concentration of copper fails to rescue this defect. (A) Clutches of embryos from a WT zebrafish and (B) from a cross of a pair of Ctr1+/- heterozygous zebrafish were imaged at 48hpf following treatment with and without 100 nM elesclomol (ES) or 100 nM copper chloride (CuCl2). Arrowheads in (B) indicate homozygous Ctr1-/- embryos with a pigmentation defect. Notably, not a single embryo with pigmentation defect was observed upon ES treatment.
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