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Fig. 3
Tdrd6a Is Required for Bb Integrity
(A) Quantification of Bb phenotypes as indicated based on dazl FISH on oocytes (examples indicated on the left, arrows indicate Bb).
(B) Surface ratio of Bbs in wt versus tdrd6a mutant oocytes (∗ indicates p value < 0.001, Wilcoxon test).
(C and D) Confocal images of Buc-eGFP-positive oocytes in tdrd6a+/− (C) and tdrd6a−/− (D) background. IHC for Tdrd6a and double smFISH was performed and displayed as indicated. Dazl and vasa signals typically do not overlap, illustrated in the line graph. Intensity for dazl (red) and vasa (cyan) signals over line a-b (see overlay), with vertical lines indicating fluorescence peaks per smFISH signal (highlighted by colored circle on top) showing transcript peaks are in a separate phase.
(E and F) Electron micrographs of Bbs of tdrd6a+/− (E) and tdrd6a−/− (F) oocytes (white dashed line). The zoom (black dashed square) is shown with (right) and without (middle) overlays that mark the Gp (yellow) and mitochondria (cyan). Scale bars, 10 μm (overview C and D), 2 μm (zoom) (C and D), and 2 μm (E and F).
See also Figure S4.
Reprinted from Developmental Cell, 46, Roovers, E.F., Kaaij, L.J.T., Redl, S., Bronkhorst, A.W., Wiebrands, K., de Jesus Domingues, A.M., Huang, H.Y., Han, C.T., Riemer, S., Dosch, R., Salvenmoser, W., Grün, D., Butter, F., van Oudenaarden, A., Ketting, R.F., Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification, 285-301.e9, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell