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Fig. 5
Dlc, dld and vegfaa-165 can rescue HSC specification in Rspo1 morphants. Expression of the HSC markers runx1 (A,D,G,J,M,P,S) and cmyb (B,E,H,K,N,Q,T), and the T lymphocyte marker rag1 (C,F,I,L,O,R,U) in the thymus were examined at 24 hpf, 36 hpf, and 96 hpf as indicated, by WISH in wild-type embryos (A-C), or embryos injected with 5 ng Rspo1 MO (D-F), or 5 ng Rspo1 MO and mRNAs encoding candidate rescue genes dlc (100 ng; G-I), dld (50 ng; J-L), dlc+dld (50 ng and 25 ng, respectively; M-O), vegfaa-165 (100 ng; P-R) or vegfaa-121 (100 ng; S-U). dlc and dld mRNA were independently able to partially rescue HSCs. Co-injection of dlc and dld caused a more complete rescue of HSCs. vegfaa-165, but not vegfaa-121, was able to rescue HSCs. Red arrowheads indicate runx1+ (A,D,G,J,M,P,S), and cmyb+ (B,E,H,K,N,Q,T) HSCs, or rag1+ T lymphocytes (C,I,L,O,R), when visible. Numbers of embryos displaying the depicted phenotype are indicated. Lateral views of the mid trunk at 160× magnification.