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Fig. S8
Direct injection of fertilized zebrafish embryos with Gfap constructs showed mutant-specific tail defects.
Fluorescent imaging showed CNS-specific expression of injected construct GfapGFP (A), GfapIDH1wt (B) and GfapR132C(C) and the corresponding bright-field images (A’-C’). D: the percentage of GFP-positive embryos with tail defects per construct. The ratio of injected embryos with tail defect were calculated based on results of three independent experiments (~100 eggs/construct/experiment). n.s: non-significant. Scale bar: 500μm.
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