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Fig. 7
RA treatment leads to the inhibition of joint cell marker expression. (A) Fish were treated with RA from 3 to 4 dpa. (B-D) ISH on longitudinal cryosections at 4 dpa/1 dot indicate hoxa13a (B), evx1 (C) and pthlha (D) expression are lost in joint cells of RA-treated fish. (B′-D″) Expression remains in joint cells (blue arrowheads) of ethanol (B′-D′) and water (B″-D″) controls. Numbers in each panel represent the number of sections with the expression pattern over the total number of sections analyzed. Scale bar: 100 μm (in B for B-D″).
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