ZFIN Image: Zhang et al., 2018, Fig. 4

Image

Figure Caption

Fig. 4

Lens fiber cell differentiation is defective in ddx39ab mutant embryos. (A) ddx39ab mutants exhibit defects in lens fiber morphogenesis. (Top) Bright-field images of the eyes from wild-type and ddx39ab mutant zebrafish embryos at 28 hpf. The scatter plot shows the diameter of individual lens fibers. (Middle) Equatorial sections through the lens center showing the organization of lens fibers; cell membrane is labeled with mRFP. Cell number was quantified on equatorial sections. (Bottom) Schematics showing the shape and arrangement of lens fiber cells. The convexity of lens fiber cells is plotted. Scale bars: 50 μm. (B,C) RNA in situ hybridization and qPCR results for lens gene expression in wild-type and ddx39ab mutant zebrafish embryos at 32 hpf. Frontal views with dorsal side to the top. (A-C) At least 20 embryos of each genotype were analyzed and representative samples are shown. All data are mean±s.e.m. n.s, not significant. **P<0.01, ***P<0.001.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development