ZFIN Image: Esancy et al., 2018, Fig. 2

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Figure Caption

Fig. 2

Trpa1b, but not Tlr7, is required for both neuronal and behavioral responses to IMQ.

(A, B) In situ hybridization in 3dpf WT larvae probing for tlr7 and trpa1b mRNA respectively. (C, D) Larval locomotor assay of 5dpf tlr7^+/+ and tlr7^-/- (C) or trpa1b^+/+and trpa1b^-/- (D) larvae. (E, F) Adult lip-rubbing behavioral assay of tlr7^+/+/tlr7^-/- (E) and trpa1b^+/+/trpa1b^-/-(F) fish. (C), (D), (E), (F), 100 μM IMQ used. ***p<0.001, **p<0.01, Student’s t-test. Bars represent mean ± s.e.m. (G, H) Representative calcium imaging traces of 3dpf trpa1b^+/+ (G) and trpa1b^-/- (H) larvae in a transgenic elavl3HuC:GCaMP5 background exposed to 100 μM IMQ, 50 μM AITC, and 1 mM 2-APB. B = blood, TG = trigeminal ganglion.

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Acknowledgments

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