Fig. 6

EtOH exacerbates dystrophy in sapje mutants. (A–D) Anterior left, dorsal top, side-mounted, 72 hpf embryos stained with phalloidin (white) to visualize actin. (A) Wildtype control embryo. Muscle fibers are attached to the MTJ. (B) sapje mutant control embryo. A few muscle fibers detach from the MTJ (white arrows). (C) Wildtype embryo treated continuously with 2% EtOH from 30 to 72 hpf. A few muscle fibers detach from the MTJ (white arrow). (D) sapje mutant embryo treated continuously with 2% EtOH from 30 to 72 hpf. Note the increased number of muscle fibers detached from the MTJ (white arrows). (E) Quantification of fiber detachment frequency in sapje mutant embryos without and with EtOH exposure. sapje mutant embryos that were treated continuously with 2% EtOH showed an average increase in fiber detachments per embryo (p = 0.06). “e” = embryo. Whiskers are 10th–90th percentiles.