|
Fig. S4
Reelin immunoreactivity in the neuropil of 3 dpf reln-/- larvae. Related to Figure 4.
(A) Immunostaining of anti-reelin (green) and DAPI (blue) on a horizontal cryosection of a 3 dpf larval tectum. Scale bar = 20μm. The yellow rectangle indicates the long-axis along which the Reelin gradient in (B) was measured.
(B) Densitometric plot of normalized anti-reelin fluorescence intensity taken from 3 different tecta at 3dpf. Data are represented as mean ± s.e.m.
(C, D) Horizontal sections of 5dpf tectum showing reelin spatial distribution detected wildtype larva (C) or a reln-/- mutant (D). The reelin protein is absent in the mutant. Scale bars = 30 μm.