Fig. 1
Smyd1a is specifically expressed in the heart and fast-twitch skeletal muscle cells. (A, B) smyd1a mRNA is strongly expressed in somatic muscle cells and both heart chambers (inset) as revealed by ISH. (B) Smyd1 specific RT-PCR confirms expression of all smyd1 transcription variants in embryonic heart tissue. (C) Smyd1a-specific ISH and immunostaining of slow-twitch skeletal muscle fibers (slow MHC) in a transversal section of a zebrafish embryo. (D) Fluorescent imaging of transgenic lines carrying either a smyd1b_tv1-gfp fusion construct or smyd1a-gfp fusion construct under the muscle specific unc45bmin-promoter in combination with co-immunostaining against sarcomeric α-Actinin (Z-disc marker).