Fig. 6

Dusp4-Mediated Repression of Fgf Signaling Is Required for Endoderm Specification

(A) Representative images of 75% epiboly zebrafish embryos injected with control MO (con MO) or dusp4 MO3, stained for sox17 by WISH. Arrowhead indicates dorsal forerunner cells for comparison of staging.

(B) As in (A), but stained for foxa2. Arrow, foxa2 expression in axial mesoderm.

(C) Quantification of endodermal progenitors in 75% epiboly embryos, injected with indicated dusp4 MOs. Means ± SD, Mann-Whitney U test; ^∗p < 0.05.

(D) qPCR on 50% epiboly embryos injected with control MO or dusp4 MOs. Means ± SEM, n ≥ 2, t test; ^∗p < 0.05.

(E) Traces of noto expression detected with FISH in 50% epiboly zebrafish embryos injected with control MO or dusp4 MO3. For each condition, n = 3. Means are shown by the lines and the shading indicates the SD.

(F) Animal views of germ ring-stage embryos, control-injected or injected with dusp4 mRNA, containing Ndr1-expressing clone and stained for lft2 and sox32.

(G) Quantification of sox32-positive cells as in (F). Means ± SD, Mann-Whitney U test; ^∗p < 0.05.

Scale bars, 100 μm. See also Figure S6.