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Fig. 2
Double labeling in situ hybridization of lhb mRNA and three types of nER. A, D and G: lhb mRNAs were labeled in green; B, E and H: the three types of nERs (esr1, esr2a and esr2b respectively) were labeled in magenta; C, F and I: in each line, lhb mRNA and nER labeling images were superimposed with NIS Elements D3.2; Nikon. lhb mRNA was found to be colocalized with esr1 and esr2a (white arrows) but not esr2b in the pituitary glands of early pubertal female zebrafish. Scale bar: 20 μm.
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Reprinted from Molecular and Cellular Endocrinology, 461, Li, G., Tang, H., Chen, Y., Yin, Y., Ogawa, S., Liu, M., Guo, Y., Qi, X., Liu, Y., Parhar, I.S., Liu, X., Lin, H., Estrogen directly stimulates LHb expression at the pituitary level during puberty in female zebrafish, 1-11, Copyright (2017) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.