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Fig. 1
Stainings for PCNA (a,b), BrdU (c) and geminin (d-f). All stainings were performed on 72 hpf embryos. (a) Sagittal section stained for PCNA. Strong staining is found in the tectum (blue arrow) and gastro-intestinal tract (black arrows). (b) Transversal section stained for PCNA. PCNA stains positive in the gut tube, swim bladder and liver. (c) Transversal section stained for BrdU. BrdU intake is prominent in intestinal cells. (d–f) Separate images of nuclear staining (DAPI) (d), mCherry (zGem signal) (e) and overlay (f). (f) mCherry signal is present in a large proportion of intestinal cells.
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