ZFIN Image: Giger et al., 2017, Fig. S6

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Figure Caption

Fig. S6

Endodermal cells internalize in the absence of YSL translation. (A) Rhodamine-dextran, alone or in combination with RNase, was injected into the YSL of embryos at the midblastula stage. Endodermal cells were then transplanted into the animal pole at the late blastula stage. (B) RNase injection induces strong developmental defects. Image on B was acquired on a stereo microscope. Two separate images (brightfield and red fluorescence) were acquired and overlaid in Photoshop. (C–F) In situ hybridization for YSL transcripts A2ML (C and D) and cb120 (E and F) in control (C and E) and RNase-injected (D and F) embryos. (G and H) Endodermal cells transplanted to the animal pole of RNase-injected embryos (G) were internalized by midgastrulation (H). (Scale bar: 10 μm.)

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA