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Fig. S4
Imaging of pancreatic islet vascularization and treatment with Pazopanib. (A) Confocal section of a 30 hpf endocrine pancreas showing β cells (ins:DsRed) completely surrounded by blood vessels (fli1a:EGFP). Vessels are not only located in contact to the peripheral surface of the pancreatic islets, but also penetrate in the inner region between islets. (B) 3D reconstruction of a 30 hpf endocrine pancreas showing blood vessels wrapped around the β cells. C) Time-lapse video of a 30 hpf pancreatic endocrine islet (red, ins:DsRed) surrounded and penetrated by blood vessels (green, fli1a:EGFP). Full 3D stacks were taken every 10 minutes over a period of 3 hours. (D) Treated larvae were subjected to 0.5 μM Pazopanib treatment for 72 h (from 2 dpf to 5 dpf). (E) Integrated density analysis of fluorescence of 5 dpf zebrafish larvae with or without Pazopanib treatment. Values represent the mean ± SEM. Asterisks indicate that expression levels are significantly different from the control: *** p<0.001. n=6 larvae for each group.