|
Fig. 4
EB3 comets originate near branch points and growth cones in sensory neuron peripheral axons. (A) RB neuron labeled with EB3-GFP and TagRFP-caax in a Clstn-1 mutant embryo. (A') Yellow box outlines area shown in time-lapse sequence, which shows a retrograde comet originating from a newly forming branch. Scale bar is 10 μm. Time is shown in min:sec. (B) In terminal peripheral axon branches the distance between the origin of retrograde EB3-GFP comets and the growth cone was measured. (C) In non-terminal peripheral axon segments the distance between the origin of retrograde EB3-GFP comets and the distal branch point was measured. (D) Histogram showing the number of retrograde comets originating at indicated distance from the growth cone. (E) Histogram showing the number of retrograde comets originating at indicated distance from branch point. (F) Retrograde comets of Clstn-1 lof originate closer to branch points than wild type (WT mean distance to branch point = 20.26 μm, n = 13 comets; Clstn-1 MO mean distance to branch point = 6.02 μm, n = 18 comets, Dunnett's post-test **p = 0.0030 Dunnett's post-test; Clstn-1−/− near BP distance = 3.55 μm, n = 18 comets, ***p = 0.0005 Dunnett's post-test; ***p = 0.0006 one-way ANOVA).