Fig. 5
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Fig. 5
Fgf24 regulates gene expression of somatic gonad cells.
(A-J) Single plane confocal micrographs of whole-mount larval gonads after fluorescent in situ hybridization detecting SGC markers (red). Nuclei labeled with DAPI (blue), germ cells labeled with Vasa (green). gata4 (A), nr5a1a (C), wt1a (E), cyp19a1a (G), and amh (I) are expressed in somatic gonad cells (SGCs) of wild-type larval gonads, whereas only wt1a can be detected in SGCs of fgf24 mutants (F). (K-M) Double fluorescent in situ hybridization and Vasa antibody staining (blue) of 12 dpf wild-type gonads (outlined by white dotted line). etv4, cyp19a1a, and amh are expressed in non-overlapping SGC domains (n = 12, 12, and 3 for K, L, and M, respectively). In E, arrows denote inner SGCs; arrowheads denote outer SGCs. A-M are sagittal optical sections with anterior to the left. Scale bars = 20 μm.