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Fig. 3
tbx6l; tbx16 double mutants have an enlarged tail bud and lack tail somites. A–C′: Brightfield images of 24-hpf live wild-type (A, A′), tbx16 (spt) mutant (B,B′), and tbx6l; tbx16 double-mutant (C,C′) embryos. tbx6l single-mutant embryos are not shown here as they are indistinguishable from wild-type embryos (see Fig. 1F,G). Black dots in the magnified views (A′–C′) indicate morphologically visible tail somites. D–F: In situ hybridization for msgn1 at the 16-somite stage. Scale bar in A (for A–C) = 250 μm. Scale bar in A′ (for A′–C′) = 100 μm. Scale bar in F (for D–F) = 50 μm.
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