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Fig. 4
Upregulation of MuRF1a leads to myofibril disarray.
(A) Schematic representation of the construct that drives cardiac-specific MuRF1a expression. (B) Murf1a expression is upregulated in the 2-day-old myl7:MuRF1a-IRES-GFP heart (right panel) compared to the wild type heart (left panel). (C) α-actinin staining in 3-day-old wild type (left panel) and transgenic (right panel) cardiomyocytes. Note that sarcomeres are disassembled in myl7:MuRF1a-IRES-GFP cardiomyocytes. (D) Live images of wild type and Tg(myl7:MuRF1a-IRES-GFP) fish at 72 hpf (left panels). Transgenic hearts are GFP positive and become dilated (inset). (E) Heart rate (HR) and atrial fractional shortening (FS) in wild type and myl7:MuRF1a-IRES-GFP embryos at 72 hpf. *p<0.05; ***p<0.001.