ZFIN Image: Gallagher et al., 2017, Fig. S4

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Figure Caption

Fig. S4

Venus reporter transcripts accumulate in pnrc2- and upf1-deficient embryos, but Venus protein expression appears normal. Embryos carrying the her1:her1-Venus^bk15 transgenic clock reporter were injected with a moderate pnrc2 sbMO dose (6 ng) or co-injected with low pnrc2 and upf1 sbMO doses (2 ng and 0.25 ng, respectively) to detect Venus transcripts (A-C) and Venus protein (D-F) at mid-segmentation stages. Representative embryos are shown in A (n=12), B (n=8), C (n=9), D (n=36), E (n=24), and F (n=27). Venus immunofluorescence panels (D-F) are at slightly higher magnification than Venus in situ panels (A-C). sbMO = splice-blocking MO; scale bar = 50 um (D-F).

Acknowledgments

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Reprinted from Developmental Biology, 429(1), Gallagher, T.L., Tietz, K.T., Morrow, Z.T., McCammon, J.M., Goldrich, M.L., Derr, N.L., Amacher, S.L., Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation, 225-239, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.