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Fig. S5
her1 transcripts are not detected in embryos homozygous for a her1 deficiency. Detection of her1 mRNA by in situ hybridization chain reaction (HCR-ISH) by confocal microscopy reveals striped her1 expression in the presomitic mesoderm (PSM) of wild-type embryos (A), mirroring what is observed by colorimetric in situ. As expected, Df(Chr05:her1,her7,ndrg3a)b567 homozygote embryos, imaged using the same laser intensity and exposure as wild-type embryos in A, lack detectable her1 expression (B). Scale bar = 50 um.
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Reprinted from Developmental Biology, 429(1), Gallagher, T.L., Tietz, K.T., Morrow, Z.T., McCammon, J.M., Goldrich, M.L., Derr, N.L., Amacher, S.L., Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation, 225-239, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.