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Fig. 4
pnrc2 is broadly expressed during segmentation stages. pnrc2 transcripts are detected during early embryonic stages and throughout segmentation stages by in situ hybridization (n>15 per time point) (A-F). As expected, there is no detectable staining with a pnrc2 sense probe (n=15/15) (data not shown). RT-PCR expression analysis for pnrc2 at 2 hours post fertilization (2 h) through mid-segmentation (18 h) is consistent with in situ detection of pnrc2 transcript (see Methods and Results) (G).
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Reprinted from Developmental Biology, 429(1), Gallagher, T.L., Tietz, K.T., Morrow, Z.T., McCammon, J.M., Goldrich, M.L., Derr, N.L., Amacher, S.L., Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation, 225-239, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.