ZFIN Image: Peng et al., 2017, Fig. 2

Image

Figure Caption

Fig. 2

cyp2r1 Gene Depletion in Zebrafish by TALENs

(A) The binding site of engineered TALENs on the cyp2r1 gene exon (E)1 (exons are in boxes). The underlined blue font indicates the sequences of the two targeting arms of TALENs, and the red font shows the restriction enzyme AluI cutting site.

(B) Mutation confirmation, as shown by the sequencing results of the transcripts of cyp2r1 gene of M1 and M2.

(C) A diagram representative of WT and two truncated mutant Cyp2r1 proteins.

(D) An agarose gel electrophoresis image with the PCR products following AluI digestion of the cyp2r1 locus of F₂ fish. +/+, WT; +/−, heterozygous; −/−, homozygous.

(E) The relative expression levels of cyp2r1 in the adult livers of mutant lines and their control siblings. ^∗∗p < 0.01, compared with the control group.

(F and G) Comparison of the morphological features of the cyp2r1^−/− fish and wild-type controls at (F) 5 dpf and (G) 100 dpf.

See also Figure S2 and Tables S1 and S2.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.