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Fig. S4
Mmp2 inhibition causes an accumulation of fibronectin in the AGM.
Related to Figure 4.
A) Exposure to SB-3CT (10μM, 32-48 hpf) increased fibronectin staining in the AGM compared to controls at 48 hpf (n≥10 embryos/condition).
B) Vibratome cross-sections show increased fibronectin staining in the posterior cardinal vein (PCV) and ventral wall of the dorsal aorta (DA) with SB-3CT treatment (32-54 hpf) compared to controls; inset magnifications of boxed regions (from left panels) show gray-scale fluorescence of fibronectin (right; n≥7 embryos/condition).
Scale bars: 30 (A) and 15μm (B).
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