ZFIN Image: Zhang et al., 2015, Fig. 5

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Figure Caption

Fig. 5

7α-25-OHC stimulation facilitates HSPC development in zebrafish. (A) Expression of ve-cadherin increases in clotrimazole-treated embryos. (B) Transition of HE to HSPCs or non-hemogenic ECs after DMSO or clotrimazole treatment beginning at 30 hpf. White asterisk indicates the same cell in different frames. (C) Quantification of HE (flk1⁺cmyb⁺, yellow cells in B) differentiating to HSPCs (flk1⁻cmyb⁺, green cells in B) or non-hemogenic ECs (flk1⁺cmyb⁻, red cells in B) (n = 5). Error bar represents SD. (D, E) runx1 expression in embryos treated with 7α-25-OHC or clotrimazole and examined at 36 hpf by WISH (D) and western blotting (E). Red arrowheads in A and D label the staining signals in AGM.

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Acknowledgments

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