Fig. 8

Fig. 8

dlx1a and dlx2a expression is wdr68-dependent and responsive to inhibition of TGF-β signaling in wdr68^{hi3812/hi3812} zebrafish.

(A-H) ISH analysis of prim-12 stage embryos raised at 32°C. A-F) dlx2a expression with red underline for anterior portion of 1^st arch and blue underline for 2^nd arch. A, C, E) lateral view. B, D, F) dorso-lateral view. A, B) DMSO-treated wildtype sibling showing normal dlx2a. C, D) DMSO-treated wdr68^{hi3812/hi3812} mutant showing loss of anterior 1^st arch dlx2a. E, F) SB431542-treated wdr68^{hi3812/hi3812} mutant showing partial rescue of anterior 1^st arch dlx2a. G, H) dlx1a expression. G) wildtype sibling showing normal dlx1a. H) wdr68^{hi3812/hi3812} mutant showing loss of anterior 1^st arch dlx1a.