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Fig. 1
Characterization of human and zebrafish RBM47. A: Diagrammatic representation of human and zebrafish RBM47 (Rbm47) proteins (not to scale). Human RBM47 gene encodes two alternatively spliced transcripts, resulting in two protein isoforms a and b. The gray box indicates the nonhomology region resulting from alternative splicing. Zebrafish rbm47 gene encodes only one isoform. All proteins possess three RRM domains. B: Sequence alignment of highly conserved regions of human and zebrafish RBM47 (Rbm47) RRMs. The zebrafish Rbm47 RRM has 90% sequence identity compared with those of the human orthologue. C: HeLa cells transfected with RBM47-GFP fusion plasmid express RBM47 in the nucleus, compared with control-transfected cells that express green fluorescent protein (GFP) throughout the cell. D: The RNA binding assay demonstrates RBM47′s ability to bind poly-A, -U, and -C RNA, while weakly binding poly-G RNA, and having no affinity for single-stranded DNA as shown in lane S. E: rbm47 is expressed ubiquitously during zebrafish embryogenesis, as shown by whole mount in situ hybridization. Scale bar = 200 µm.