ZFIN Image: Thambyrajah et al., 2016, Fig. S4

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Figure Caption

Fig. S4

Most GFP-positive cells that patrol the head tissues of qmc551 transgenic embryos do not express markers of mature macrophages and neutrophil granulocytes. (A) Live embryos that carry the qmc551 gene trap and the macrophage reporter transgene mpeg1:mCherry display numerous GFP (green arrow) and mCherry (red arrow) single-positive cells, but hardly any double-positive cells (yellow arrow). (B) Fixed qmc551 transgenic embryos stained for the mature neutrophil marker Sudan Black and immunostained for GFP harbor many cells that are single-positive for GFP (green arrow) and for Sudan Black (black in the original image; red in the pseudocolored image; red arrow). All images show ventral views of the head region of the embryo, with anterior to the left.

Acknowledgments

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Reprinted from Developmental Biology, 417(1), Thambyrajah, R., Ucanok, D., Jalali, M., Hough, Y., Wilkinson, R.N., McMahon, K., Moore, C., Gering, M., A gene trap transposon eliminates haematopoietic expression of zebrafish Gfi1aa, but does not interfere with haematopoiesis, 25-39, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.