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Fig. S1
In the gene trap line qmc551, GFP is expressed in primitive erythrocytes and in endothelial cells of the ventral wall of the dorsal aorta. This figure supports data shown in Fig. 1. (A) Single color and merged confocal images of the trunk of a qmc551;gata1:dsRed double transgenic embryo after fluorescent GFP and dsRed immunostaining. (B) Single color and merged confocal images of a qmc551;flk1:tdTom double transgenic embryo after fluorescent immunostaining for GFP and tdTom. (C) Single color and merged confocal images of a live qmc551;csl:cer double transgenic embryo. Note that prRBCs in circulation appear as short lines in the confocal image in (C), while stationary cells are round. Images were taken at single cell resolution on 2 (A), 1.8 (B) and 2.5 (C) µm optical slices. Times given on the panels represent hours and minutes after fertilization. All images show embryos with anterior to the left and dorsal up. Red arrows - prRBCs; red arrowheads - prRBC progenitors trapped in the mesenchyme; green arrows - ECs in the vDA.
Reprinted from Developmental Biology, 417(1), Thambyrajah, R., Ucanok, D., Jalali, M., Hough, Y., Wilkinson, R.N., McMahon, K., Moore, C., Gering, M., A gene trap transposon eliminates haematopoietic expression of zebrafish Gfi1aa, but does not interfere with haematopoiesis, 25-39, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.