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Fig. 2
Phenotype of spiel ohne grenzen (spg) mutants on day 2 of development. (A,B) DIC image of wild-type (A) and spgm216 mutant (B) embryos at 30 hpf. Arrowhead indicates the position of midbrain-hindbrain boundary. (C,D) Expression of engrailed-2 in wild-type (C) and strong spgm216 mutant (D) embryos at 28 hpf. Weaker spgm216 mutants retain a small dorsal patch of engrailed-2 expression. (E,F) Expression of dlx2 and hlx1 in wild-type (E) and spgm216 mutant (F) embryos at 28 hpf. Arrowhead indicates position of the prospective tectum.
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