ZFIN Image: Song et al., 2016, Fig. S1

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Figure Caption

Fig. S1

mValRS^ED mRNA injection into zebrafish results in cell death and p53 activation. A) Acridine orange (AO) staining at 1 dpf of zebrafish uninjected (n=51), or injected with either mValRS^WT mRNA (n=56) or mValRS^ED mRNA (n=63). The red arrows show points of cell death. Zebrafish are aligned vertically based on cell death severity and morphology; normal (top), increased cell death (middle), and increased cell death with abnormal morphology (bottom). Scale bar represents 500 µm. B) Quantification of acridine orange staining per fish. Bars represent mean ± SEM. C) Uninjected (n=43), mValRS^WT mRNA injected (n=44), or mValRS^ED mRNA injected (n=35) p53^zdf1/zdf1 zebrafish were stained with acridine orange at 1 dpf. The red arrows show points of cell death. Zebrafish are aligned based on cell death severity. Scale bar represents 500 µm. D) Quantification of acridine orange staining. Bars represent mean ± SEM. RT-PCR results of p53 downstream markers p21, gadd45, p53R2, bax and mdm2 in E) p53^+/+ or F) p53^zdf1/zdf1 zebrafish injected with either mValRS^WT or mValRS^ED mRNA.

Acknowledgments

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