ZFIN Image: Xu et al., 2016, Fig. S3

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Figure Caption

Fig. S3

Colonization of the Optic Tectum by Microglial Precursor Depends on Apoptotic Neurons, related to Figure 3

(A) Acriding Orange (AO) staining (green) reveals the accumulation of apoptotic neurons in the optic tectum of the zebrafish brain from 2 dpf to 3 dpf and the subsequent reduction of apoptotic neurons at 6 dpf. The optic tectum is indicated by dashed lines.

(B) WISH shows a robust expression of bcl-2 in the brain of the 3 dpf Tg(Xla.Tubb:bcl-2) embryos.

(C) mfap4 WISH staining shows normal development of peripheral macrophages in Tg(Xla.Tubb:bcl-2) embryo during early development.

(D) Photo-converted coro1a-Kaede⁺ microglia (red signals) in the optic tectum (white dashed lines) at 3 dpf and 6 dpf .

(E) Quantification of Photo-converted coro1a-Kaede⁺ microglia at 3 dpf and 6 dpf . n=4 for 3 dpf and 6 dpf embryos. Error bars represent mean SEM.

Acknowledgments

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Reprinted from Developmental Cell, 38(2), Xu, J., Wang, T., Wu, Y., Jin, W., Wen, Z., Microglia Colonization of Developing Zebrafish Midbrain Is Promoted by Apoptotic Neuron and Lysophosphatidylcholine, 214-22, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell