ZFIN Image: Venero Galanternik et al., 2015, Fig. S3

Image

Figure Caption

Fig. S3

Pharmacological inhibition of HS recapitulates extl3/ext2 mutants. Related to Figures 2-4.

(A-C′) The ectopic pea3 expression surrounding the primordium is Fgf dependent in extl3/ext2 mutants (B-B′) and NaClO3-treated embryos (C-C′). (D-F′′) The ectopic pea3 expression is restricted to cells immediately surrounding the primordium in extl3/ext2 mutants (E-E′′) and NaClO3-treated embryos (F-F′′) as demonstrated by double labeling with ClaudinB:GFP and pea3. (G-H′′) 3G10 labels HS chains and its persistence in NaClO3 treated embryos shows that only HS chain modifications are lost (See Table S1). (I-W′) Time course of the Fgf targets pea3 and dkk1b and the Wnt/β-catenin target lef1 in DMSO controls compared to NaClO3-treated embryos. Similar to extl3/ext2 mutants, lef1 expands only as pea3 and dkk1b are gradually downregulated after NaClO3- treatment.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.