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Fig. 1
Efficiency of msrb3 morpholinos. (A) WISH at 12 hpf showed that msrb3 was widely expressed in embryos (a). At 26 hpf msrb3 was highly expressed in the inner ear of embryos (b). (B) Morpholino antisense oligonucleotides. (C) Primers designed to measure efficiency of In2MO (179 bp and 299 bp products). (D) Primers designed to measure efficiency of Ex2MO (less 163 bp product). (E) Morpholino efficiency: two msrb3 morpholinos could interfere with normal mRNA splicing (compare lane 1 to lane 2 and lane 3 to lane 4).
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Full text @ Int. J. Dev. Biol.