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Fig. 4
Characterization of the function of LPA3 during primitive erythropoiesis in zebrafish embryos.
Zebrafish embryos were immersed in (a) the LPA1/3 antagonist Ki16425 or (b) the LPA3 agonist 2S-OMPT for 48 h. O-dianisidine staining was classified as normal, moderate, or severe based on the expression level of embryonic blood in the CHT region. The right-hand panel shows the quantitative results. (c) Whole-mount in situ hybridization showed the mRNA expression of α-A1, β-E1 globin and ikaros in zLPAR3 tMO-injected or wild type embryos.
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