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Fig. S4
Injection of optimally salt-solubilized RNPs into the yolk decreases mutagenesis efficiency to levels suitable for germline transgenesis. (A) Heterozygous ubi:EGFP control embryo. (B) EGFP mutagenesis using Cas9p and EGFP sgRNA injected into the yolk instead of the cell at the one cell-stage; inserts show brightfield image of fluorescent view. (c) Relative EGFP intensity from cell versus yolk injections.
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